pGST1-yVps27-VHS
(Plasmid
#44417)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 44417 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGST1
- Backbone size w/o insert (bp) 5000
- Total vector size (bp) 5447
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameVps27-VHS (2-150)
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)447
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Entrez GeneVPS27 (a.k.a. YNR006W, DID7, GRD11, SSV17, VPL23, VPT27)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer 5GEX (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGST1-yVps27-VHS was a gift from James Hurley (Addgene plasmid # 44417 ; http://n2t.net/addgene:44417 ; RRID:Addgene_44417) -
For your References section:
VHS domains of ESCRT-0 cooperate in high-avidity binding to polyubiquitinated cargo. Ren X, Hurley JH. EMBO J. 2010 Mar 17;29(6):1045-54. Epub 2010 Feb 11. 10.1038/emboj.2010.6 PubMed 20150893