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Addgene

pGST2-STAM1-VHS
(Plasmid #44414)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44414 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGST2
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 5429
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    STAM1-VHS
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    429
  • Entrez Gene
    STAM (a.k.a. STAM-1, STAM1)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer 5GEX
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGST2-STAM1-VHS was a gift from James Hurley (Addgene plasmid # 44414 ; http://n2t.net/addgene:44414 ; RRID:Addgene_44414)

  • For your References section:

    VHS domains of ESCRT-0 cooperate in high-avidity binding to polyubiquitinated cargo. Ren X, Hurley JH. EMBO J. 2010 Mar 17;29(6):1045-54. Epub 2010 Feb 11. 10.1038/emboj.2010.6 PubMed 20150893
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