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Addgene

GFP-MAP1B
(Plasmid #44396)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44396 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4731
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MAP1B
  • Alt name
    microtubule-associated protein 1B
  • Species
    M. musculus (mouse)
  • GenBank ID
    NM_008634.2 NP_032660.2
  • Entrez Gene
    Map1b (a.k.a. A230055D22, LC1, MAP5, Mtap-5, Mtap1b, Mtap5)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer EBV_rev_primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-MAP1B was a gift from Phillip Gordon-Weeks (Addgene plasmid # 44396 ; http://n2t.net/addgene:44396 ; RRID:Addgene_44396)

  • For your References section:

    Nonprimed and DYRK1A-primed GSK3 beta-phosphorylation sites on MAP1B regulate microtubule dynamics in growing axons. Scales TM, Lin S, Kraus M, Goold RG, Gordon-Weeks PR. J Cell Sci. 2009 Jul 15;122(Pt 14):2424-35. doi: 10.1242/jcs.040162. Epub 2009 Jun 23. 10.1242/jcs.040162 PubMed 19549690
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