pMMB66EH-aphA
(Plasmid
#44392)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 44392 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMMB66EH
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Backbone manufacturerAATC
- Backbone size w/o insert (bp) 8807
- Total vector size (bp) 9323
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Modifications to backbonenone
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsCan also grow in Vibrio cholerae at 30 degrees.
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameAphA
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Alt nameVC2647 (N16961)
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SpeciesVibrio cholerae
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Insert Size (bp)542
- Promoter tac
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer GGA ATT GTG AGC GGA TAA CA
- 3′ sequencing primer GAT TTA ATC TGT ATC AGG (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMMB66EH-aphA was a gift from Victor DiRita (Addgene plasmid # 44392 ; http://n2t.net/addgene:44392 ; RRID:Addgene_44392) -
For your References section:
Small-molecule inhibitors of toxT expression in Vibrio cholerae. Anthouard R, DiRita VJ. MBio. 2013 Aug 6;4(4). pii: e00403-13. doi: 10.1128/mBio.00403-13. 10.1128/mBio.00403-13 PubMed 23919997
