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pACYC-PLlambda-alkSm4-3
(Plasmid #44383)

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Item Catalog # Description Quantity Price (USD)
Plasmid 44383 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pACYC
  • Backbone size w/o insert (bp) 3984
  • Total vector size (bp) 6633
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    AlkSm4-3
  • Species
    Pseudomonas putida
  • Insert Size (bp)
    2649
  • Mutation
    Q410I, S470T, K546E, E775D
  • Promoter PLlambda

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer p15A-F (see manuscript for additional)
  • 3′ sequencing primer pBRrevBam
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACYC-PLlambda-alkSm4-3 was a gift from Hal Alper (Addgene plasmid # 44383 ; http://n2t.net/addgene:44383 ; RRID:Addgene_44383)

  • For your References section:

    Evolution of an alkane-inducible biosensor for increased responsiveness to short-chain alkanes. Reed B, Blazeck J, Alper H. J Biotechnol. 2012 Apr 15;158(3):75-9. doi: 10.1016/j.jbiotec.2012.01.028. Epub 2012 Feb 2. 10.1016/j.jbiotec.2012.01.028 PubMed 22326628
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