pZE-PalkB-GFP
(Plasmid #44381)

• Depositing Lab: Hal Alper
• Publication: Reed et al J Biotechnol. 2012 Apr 15;158(3):75-9. doi: 10.1016/j.jbiotec.2012.01.028. Epub 2012 Feb 2.

Backbone

• Vector backbone: pZE
• Backbone size w/o insert (bp): 2048
• Total vector size (bp): 3123
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: GFP
• Insert Size (bp): 756
• Promoter: PalkB

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: see manuscript
• 3′ sequencing primer: rrnB-T1-term-Rev

Gene/Insert 2

• Gene/Insert name: PalkB
• Species: Pseudomonas putida
• Insert Size (bp): 290
• Promoter: PalkB

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: AatII (not destroyed)
• 3′ cloning site: KpnI (not destroyed)
• 5′ sequencing primer: see manuscript
• 3′ sequencing primer: GFP-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA