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Addgene

pUC-Leum
(Plasmid #44364)

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Item Catalog # Description Quantity Price (USD)
Plasmid 44364 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pUC19
  • Backbone size w/o insert (bp) 2623
  • Total vector size (bp) 2803
  • Modifications to backbone
    None
  • Vector type
    Bacterial Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Leum promoter
  • Species
    Yarrowia lipolytica
  • Insert Size (bp)
    129
  • Mutation
    N/A
  • Promoter Leum promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SphI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer m13_forward20_primer
  • 3′ sequencing primer m13_reverse_primer
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUC-Leum was a gift from Hal Alper (Addgene plasmid # 44364 ; http://n2t.net/addgene:44364 ; RRID:Addgene_44364)

  • For your References section:

    Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Blazeck J, Liu L, Redden H, Alper H. Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16. 10.1128/AEM.05763-11 PubMed 21926196
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