pFIN-RK-GFP-mIRBP156-CHER-WPRE
(Plasmid #44351)

• Depositing Lab: Susan Semple-Rowland
• Publication: Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

Backbone

• Vector backbone: pTY
• Backbone manufacturer: LJ Chang
• Backbone size w/o insert (bp): 8766
• Total vector size (bp): 10905
• Modifications to backbone: Final pFIN backbone: Added cPPT-FLAP sequence upstream of MCS in pTY to create pTYF backbone (Coleman et al 2002 Physiol Genomics 12:221-228). Added 2x250 chicken β-globin HS4 core sequence into 3'LTR of pTYF backbone to create pFIN (Semple-Rowland et al 2007 Mol Vis 13:2001-2011).
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: RK-GFP
• Alt name: human rhodopsin kinase promoter
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 1027
• Promoter: human rhodopsin kinase (pos 2554-2850)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Gene/Insert 2

• Gene/Insert name: mIRBP156-CHER
• Alt name: mouse interphotoreceptor retinal binding protein promoter
• Alt name: mCherry
• Species: M. musculus (mouse), Synthetic
• Insert Size (bp): 1104
• Promoter: mouse interphotoreceptor retinal binding protein (pos 3589-3897)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The IRBP promoter was amplified from BP1783-EGFP kindly provided by Jeffrey Boatright, Emory University.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry