pFIN-XOPS-tdTOM-MOPS-GFP
(Plasmid #44347)

• Depositing Lab: Susan Semple-Rowland
• Publication: Semple-Rowland et al Mol Vis. 2010 May 27;16:916-34.

Backbone

• Vector backbone: pTY
• Backbone manufacturer: LJ Chang
• Backbone size w/o insert (bp): 8760
• Total vector size (bp): 11600
• Modifications to backbone: Final pFIN backbone: Added cPPT-FLAP sequence upstream of MCS in pTY to create pTYF backbone (Coleman et al 2002 Physiol Genomics 12:221-228). Added 2x250 chicken β-globin HS4 core sequence into 3'LTR of pTYF backbone to create pFIN (Semple-Rowland et al 2007 Mol Vis 13:2001-2011).
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: XOPS-tdTOM
• Alt name: Xenopus Opsin Promoter
• Alt name: tdTomato
• Species: X. laevis (frog), Synthetic
• Insert Size (bp): 2869
• Promoter: Xenopus Opsin (XOPS) (pos 3131-4495)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Gene/Insert 2

• Gene/Insert name: MOPS-GFP
• Alt name: mouse opsin promoter
• Alt name: GFP
• Species: M. musculus (mouse), Synthetic
• Insert Size (bp): 1401
• Promoter: mouse opsin promoter (pos 6073-6549)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Resource Information

• A portion of this plasmid was derived from a plasmid made by: XOPS1.3 promoter (1370 bp)was amplified from XOPS1.3p44/GFP obtained from Dr. Barry Knox. MOPS promoter was amplified from pTR-MOPS500-GFP (UF5-mops) kindly provided by Dr. William Hauswirth.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry