Skip to main content
Addgene

pMX-mTAF4b
(Plasmid #44345)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44345 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pMX
  • Total vector size (bp) 7544
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Standard growth
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TAF4b
  • Alt name
    TBP-associated factor 4b
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2550
  • GenBank ID
    NM_001100449.1
  • Entrez Gene
    Taf4b (a.k.a. 105kDa, 2610524B04Rik, 4932409F03Rik, AW987595, TAFII-105, TAFII105, Taf2c2)
  • Promoter LTR
  • Tag / Fusion Protein
    • flag and SBP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Not1 (not destroyed)
  • 3′ cloning site Sal1 (not destroyed)
  • 5′ sequencing primer pBMN_5_primer
  • 3′ sequencing primer -
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is a 5aa deletion in the full plasmid seq compared to NM_001100449.1.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMX-mTAF4b was a gift from Marc Timmers (Addgene plasmid # 44345 ; http://n2t.net/addgene:44345 ; RRID:Addgene_44345)

  • For your References section:

    A central role for TFIID in the pluripotent transcription circuitry. Pijnappel WW, Esch D, Baltissen MP, Wu G, Mischerikow N, Bergsma AJ, van der Wal E, Han DW, Bruch Hv, Moritz S, Lijnzaad P, Altelaar AF, Sameith K, Zaehres H, Heck AJ, Holstege FC, Scholer HR, Timmers HT. Nature. 2013 Mar 28;495(7442):516-9. doi: 10.1038/nature11970. Epub 2013 Mar 17. 10.1038/nature11970 PubMed 23503660
Related items:
Commonly requested with: