pMX_hTAF5
(Plasmid
#44336)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 44336 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMX
- Total vector size (bp) 7010
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Growth instructionsStandard
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameTAF5
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Alt nameTBP-associated factor 5
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SpeciesH. sapiens (human)
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Insert Size (bp)2403
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GenBank IDNM_006951.3
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Entrez GeneTAF5 (a.k.a. TAF(II)100, TAF2D, TAFII-100, TAFII100)
- Promoter LTR
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Pac1 (not destroyed)
- 3′ cloning site Sal1 (not destroyed)
- 5′ sequencing primer pBMN_5_primer
- 3′ sequencing primer - (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMX_hTAF5 was a gift from Marc Timmers (Addgene plasmid # 44336 ; http://n2t.net/addgene:44336 ; RRID:Addgene_44336) -
For your References section:
A central role for TFIID in the pluripotent transcription circuitry. Pijnappel WW, Esch D, Baltissen MP, Wu G, Mischerikow N, Bergsma AJ, van der Wal E, Han DW, Bruch Hv, Moritz S, Lijnzaad P, Altelaar AF, Sameith K, Zaehres H, Heck AJ, Holstege FC, Scholer HR, Timmers HT. Nature. 2013 Mar 28;495(7442):516-9. doi: 10.1038/nature11970. Epub 2013 Mar 17. 10.1038/nature11970 PubMed 23503660