pUC-UAS1B16-Leum
(Plasmid #44324)

• Depositing Lab: Hal Alper
• Publication: Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):7905-14. doi: 10.1128/AEM.05763-11. Epub 2011 Sep 16.

Backbone

• Vector backbone: pUC19
• Backbone size w/o insert (bp): 2623
• Total vector size (bp): 4565
• Modifications to backbone: None
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: UAS1B16-Leum promoter
• Species: Yarrowia lipolytica
• Insert Size (bp): 1942
• Mutation: N/A
• Promoter: UAS1B16-Leum

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BstBI (not destroyed)
• 3′ cloning site: AscI (not destroyed)
• 5′ sequencing primer: m13_forward20_primer
• 3′ sequencing primer: m13_reverse_primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

A 140-bp minimal leucine promoter, Leum, from plasmid pMCSCen1 that was inserted into pUC19 with SphI/HindIII to form pUC-Leum. A UAS1B oligonucleotide was then inserted into pUC-Leum with SalI-HF/SphI-HF to form pUC-UAS1B1-Leum-No5′3′.