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pET-28a-hPKM2
(Plasmid #44242)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44242 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET-28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5400
  • Total vector size (bp) 7100
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Pyruvate Kinase M2
  • Alt name
    PKM2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1700
  • Entrez Gene
    PKM (a.k.a. CTHBP, HEL-S-30, OIP3, PK3, PKM2, TCB, THBP1, p58)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7 Promoter Primer
  • 3′ sequencing primer T7 Terminator Primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-28a-hPKM2 was a gift from Lewis Cantley & Matthew Vander Heiden (Addgene plasmid # 44242 ; http://n2t.net/addgene:44242 ; RRID:Addgene_44242)

  • For your References section:

    Pyruvate kinase M2 is a phosphotyrosine-binding protein. Christofk HR, Vander Heiden MG, Wu N, Asara JM, Cantley LC. Nature. 2008 Mar 13;452(7184):181-6. doi: 10.1038/nature06667. 10.1038/nature06667 PubMed 18337815
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