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Addgene

pLHCX-FLAG-mPKM2
(Plasmid #44239)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44239 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLHCX
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 6800
  • Total vector size (bp) 8400
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Pyruvate Kinase M2
  • Alt name
    PKM2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1600
  • GenBank ID
    NM_011099.3
  • Entrez Gene
    Pkm (a.k.a. Pk-2, Pk-3, Pk3, Pkm2)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HpaI (not destroyed)
  • 3′ cloning site ClaI (not destroyed)
  • 5′ sequencing primer 5'-AGCTCGTTTAGTGAACCGTCAGATC-3'
  • 3′ sequencing primer 5'-ACCTACAGGTGGGGTCTTTCATTCCC-3'
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLHCX-FLAG-mPKM2 was a gift from Lewis Cantley & Matthew Vander Heiden (Addgene plasmid # 44239 ; http://n2t.net/addgene:44239 ; RRID:Addgene_44239)

  • For your References section:

    The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth. Christofk HR, Vander Heiden MG, Harris MH, Ramanathan A, Gerszten RE, Wei R, Fleming MD, Schreiber SL, Cantley LC. Nature. 2008 Mar 13;452(7184):230-3. doi: 10.1038/nature06734. 10.1038/nature06734 PubMed 18337823
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