pRCIscript-crhr2_TAL1B
(Plasmid
#44237)
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Depositing Labs
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 44237 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneRCIscript-GoldyTALEN
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Backbone manufacturerAddgene plasmid 38142
- Backbone size w/o insert (bp) 4651
- Total vector size (bp) 6147
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Vector typemRNA transcription vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecrhr2 pTAL scaffold 1B
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SpeciesD. rerio (zebrafish)
- Promoter T3
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that some of the NN RVDs of this TALEN plasmid, which recognize G nucleotides were actually made with NK RVDs (which also recognize G nucleotides). This alters a single amino acid within the RVD.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRCIscript-crhr2_TAL1B was a gift from Karl Clark & Stephen Ekker (Addgene plasmid # 44237 ; http://n2t.net/addgene:44237 ; RRID:Addgene_44237) -
For your References section:
In vivo genome editing using a high-efficiency TALEN system. Bedell VM, Wang Y, Campbell JM, Poshusta TL, Starker CG, Krug RG 2nd, Tan W, Penheiter SG, Ma AC, Leung AY, Fahrenkrug SC, Carlson DF, Voytas DF, Clark KJ, Essner JJ, Ekker SC. Nature. 2012 Nov 1;491(7422):114-8. doi: 10.1038/nature11537. Epub 2012 Sep 23. 10.1038/nature11537 PubMed 23000899
Map uploaded by the depositor.
