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Addgene

pECFP(C1)-PP1alpha
(Plasmid #44233)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44233 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pECFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5693
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PP1A
  • Alt name
    PPP1CA
  • Alt name
    PPP1CA protein phosphatase 1, catalytic subunit, alpha isozyme
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    993
  • GenBank ID
    NM_002708
  • Entrez Gene
    PPP1CA (a.k.a. PP-1A, PP1A, PP1alpha, PPP1A)
  • Promoter CMV
  • Tag / Fusion Protein
    • CFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer TGGTCCTGCTGGAGTTCGTGACC
  • 3′ sequencing primer GTTTGGACAAACCACAACTAGAATGCAG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pECFP(C1)-PP1alpha was a gift from Angus Lamond & Laura Trinkle-Mulcahy (Addgene plasmid # 44233 ; http://n2t.net/addgene:44233 ; RRID:Addgene_44233)

  • For your References section:

    Dynamic targeting of protein phosphatase 1 within the nuclei of living mammalian cells. Trinkle-Mulcahy L, Sleeman JE, Lamond AI. J Cell Sci. 2001 Dec;114(Pt 23):4219-28. PubMed 11739654
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