pEGFP(C1)-PP1alpha
(Plasmid #44224)

• Depositing Labs: Angus Lamond, Laura Trinkle-Mulcahy
• Publication: Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5693
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PP1A
• Alt name: PPP1CA
• Alt name: PPP1CA protein phosphatase 1, catalytic subunit, alpha isozyme
• Species: H. sapiens (human)
• Insert Size (bp): 993
• GenBank ID: NM_002708
• Entrez Gene: PPP1CA (a.k.a. PP-1A, PP1A, PP1alpha, PPP1A)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: TGGTCCTGCTGGAGTTCGTGACC
• 3′ sequencing primer: GTTTGGACAAACCACAACTAGAATGCAG

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry