CBF:H2B-Venus
(Plasmid #44211)

• Depositing Lab: Anna-Katerina Hadjantonakis
• Publication: Nowotschin et al BMC Dev Biol. 2013 Apr 25;13:15. doi: 10.1186/1471-213X-13-15.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: clontech
• Backbone size w/o insert (bp): 4540
• Total vector size (bp): 4877
• Modifications to backbone: Backbone vector was pEGFP-N1 containing H2B-Venus instead of EGFP. This vector was then cut with AseI and NheI in order to excise the pCMV promoter. This cut vector was then ligated with a PCR amplified CBFRE insert obtained from addgene plasmid #17705 subcloned in the AseI and NheI sites.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CBF1-Responsive-Element
• Alt name: CBFRE
• Species: Synthetic
• Insert Size (bp): 337

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AseI (not destroyed)
• 3′ cloning site: NheI (not destroyed)
• 5′ sequencing primer: CBFRE AseI Fw
• 3′ sequencing primer: SV40 NheI Rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: insert CBFRE was amplified from addgene plasmid #17705
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry