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Addgene

BC-GPRC
(Plasmid #44199)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44199 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Barcode
  • Backbone manufacturer
    unknown
  • Backbone size w/o insert (bp) 7500
  • Total vector size (bp) 8200
  • Modifications to backbone
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    human GPR56 C-terminal fragment
  • Alt name
    GPR56 CTF
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    964
  • Mutation
    C-terminal fragment of GPR56 starting at amino acid 383, right after the cleavage site in the GPS motif
  • GenBank ID
    NM_001145770.1 NM_001145771.1
  • Entrez Gene
    ADGRG1 (a.k.a. BFPP, BPPR, GPR56, TM7LN4, TM7XN1)
  • Promoter LTR
  • Tag / Fusion Protein
    • none

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BC-GPRC was a gift from Lei Xu (Addgene plasmid # 44199 ; http://n2t.net/addgene:44199 ; RRID:Addgene_44199)
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