pcDNA3.1-Alpha-0 Dronpa
(Plasmid
#44193)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 44193 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5428
- Total vector size (bp) 6493
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameG protein alpha 0
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SpeciesR. norvegicus (rat)
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Insert Size (bp)1065
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MutationIntroduced KpnI and SpeI sites between 92nd and 93rd amino acids of alpha 0
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GenBank IDNM_017327.1
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Entrez GeneGnao1 (a.k.a. Gnao, Hg1g, RATBPGTPC)
- Promoter CMV
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Tag
/ Fusion Protein
- Dronpa
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoR1 (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T7 primer
- 3′ sequencing primer BGH primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Dronpa was inserted between amino acid 92 and 93 using the KpnI and SpeI sites which were engineered between these sites.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3.1-Alpha-0 Dronpa was a gift from Narasimhan Gautam (Addgene plasmid # 44193 ; http://n2t.net/addgene:44193 ; RRID:Addgene_44193) -
For your References section:
Shuttling of G protein subunits between the plasma membrane and intracellular membranes. Chisari M, Saini DK, Kalyanaraman V, Gautam N. J Biol Chem. 2007 Aug 17;282(33):24092-8. Epub 2007 Jun 18. 10.1074/jbc.M704246200 PubMed 17576765
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/60/39/ee2f0f08-8516-11e2-ae49-003048dd6500.pdf.940x940_q85_autocrop.png)