Skip to main content
Addgene

pcDNA3.1-Alpha-0 Dronpa
(Plasmid #44193)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44193 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5428
  • Total vector size (bp) 6493
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    G protein alpha 0
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1065
  • Mutation
    Introduced KpnI and SpeI sites between 92nd and 93rd amino acids of alpha 0
  • GenBank ID
    NM_017327.1
  • Entrez Gene
    Gnao1 (a.k.a. Gnao, Hg1g, RATBPGTPC)
  • Promoter CMV
  • Tag / Fusion Protein
    • Dronpa

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer T7 primer
  • 3′ sequencing primer BGH primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Dronpa was inserted between amino acid 92 and 93 using the KpnI and SpeI sites which were engineered between these sites.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-Alpha-0 Dronpa was a gift from Narasimhan Gautam (Addgene plasmid # 44193 ; http://n2t.net/addgene:44193 ; RRID:Addgene_44193)

  • For your References section:

    Shuttling of G protein subunits between the plasma membrane and intracellular membranes. Chisari M, Saini DK, Kalyanaraman V, Gautam N. J Biol Chem. 2007 Aug 17;282(33):24092-8. Epub 2007 Jun 18. 10.1074/jbc.M704246200 PubMed 17576765
Related items: