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Addgene

Syntaxin-4 d24
(Plasmid #44189)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44189 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA4/TO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5100
  • Modifications to backbone
    added myc tag
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Syntaxin-4
  • Alt name
    Stx4
  • Alt name
    Syn4
  • Species
    H. sapiens (human)
  • Mutation
    deleted first 24 amino acids
  • Tags / Fusion Proteins
    • 2xMyc (C terminal on backbone)
    • His (C terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

double myc tag followed by hexa-histidine tag

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Syntaxin-4 d24 was a gift from Thomas Weimbs (Addgene plasmid # 44189 ; http://n2t.net/addgene:44189 ; RRID:Addgene_44189)

  • For your References section:

    Basolateral sorting of syntaxin 4 is dependent on its N-terminal domain and the AP1B clathrin adaptor, and required for the epithelial cell polarity. Reales E, Sharma N, Low SH, Folsch H, Weimbs T. PLoS One. 2011;6(6):e21181. doi: 10.1371/journal.pone.0021181. Epub 2011 Jun 15. 10.1371/journal.pone.0021181 PubMed 21698262
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