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Atg17-Atg31-Atg29-pST39 (Auto 16)
(Plasmid #44174)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44174 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pST39
  • Backbone size w/o insert (bp) 2800
  • Total vector size (bp) 5434
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    Atg17
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1254
  • Mutation
    none

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site StuI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Atg31
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    627
  • Mutation
    none

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer M13-F20
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    Atg29
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    753
  • Mutation
    none
  • Tag / Fusion Protein
    • HIS (N terminal on insert)

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site MluI (not destroyed)
  • 5′ sequencing primer Unknown
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Atg17-Atg31-Atg29-pST39 (Auto 16) was a gift from James Hurley (Addgene plasmid # 44174 ; http://n2t.net/addgene:44174 ; RRID:Addgene_44174)

  • For your References section:

    Architecture of the Atg17 complex as a scaffold for autophagosome biogenesis. Ragusa MJ, Stanley RE, Hurley JH. Cell. 2012 Dec 21;151(7):1501-12. doi: 10.1016/j.cell.2012.11.028. Epub 2012 Dec 6. 10.1016/j.cell.2012.11.028 PubMed 23219485
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