pCI-M2
(Plasmid #44170)

• Depositing Lab: John Olsen
• Publication: Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 10.1002/jgm.2695.

Backbone

• Vector backbone: pCI
• Backbone manufacturer: Promega Corporation
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 4307
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Influenza A virus M2 matrix gene
• Alt name: M2 protein
• Alt name: M2 ion channel
• Species: Influenza virus (A/chicken/Rostock/8/34)
• Insert Size (bp): 300
• GenBank ID: M55474.1
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr Ora Weisz (Renal-Electrolyte Division, University of Pittsburgh School of Medicine, Pittsburgh, PA)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry