pNAHA
(Plasmid #44169)

• Depositing Lab: John Olsen
• Publication: Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 10.1002/jgm.2695.

Backbone

• Vector backbone: pVITRO2-mcs
• Backbone manufacturer: InvivoGen
• Backbone size w/o insert (bp): 6321
• Total vector size (bp): 9434
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Hygromycin, 200 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: hemagglutinin gene
• Alt name: influenza HA
• Species: Influenza A/FPV/Rostock/1934, subtype H7
• Insert Size (bp): 1742
• GenBank ID: M24457.1
• Promoter: CMV enhancer/human Ferritin light chain promoter

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: SgrAI (destroyed during cloning)
• 3′ cloning site: XhoI (destroyed during cloning)
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Gene/Insert 2

• Gene/Insert name: neuraminidase gene
• Alt name: influenza NA
• Species: influenza A/Puerto Rico/8/34, subtype N1
• Insert Size (bp): 1362
• Mutation: T55A
• GenBank ID: CY033579.1
• Promoter: SV40 enhancer/human ferritin heavy chain promoter

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: MluI (not destroyed)
• 3′ cloning site: AvrII (not destroyed)
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The hemagglutinin gene was obtained from Dr Francois-Loic Cosset (Laboratoire de Vectorologie Retrovirale et Therapie Genique, Lyon, France)
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that a T55A mutation was identified in the N1 sequence.