pEV53D
(Plasmid #44168)

• Depositing Lab: John Olsen
• Publication: Patel et al J Gene Med. 2013 Jan;15(1):51-62. doi: 10.1002/jgm.2695.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2900
• Total vector size (bp): 10249
• Modifications to backbone: Removed Neo gene from pcDNA3.1
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: May grow better in E. coli strain HB101.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Equine infectious anemia virus (EIAV) gag-pol,rev, and S2
• Species: Genes from Wyoming strain EIAV
• Insert Size (bp): 5200
• Mutation: A131T mutation in gag; Deletion of viral LTR sequences, deletion of viral packaging signal, deletion of first exon of tat gene, extensive deletion of env gene
• GenBank ID: M87581
• Promoter: CMV

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Dr Frederick Fuller (North Carolina State University, Raleigh, NC, USA).
• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A131T mutation in gag compared to GenBank reference sequence M87581