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Addgene

pcDNA Myc-mMEKK2
(Plasmid #44158)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44158 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Mekk2
  • Alt name
    mitogen-activated protein kinase kinase kinase 2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1800
  • Entrez Gene
    Map3k2 (a.k.a. 9630061B06Rik, AI585793, Mekk2, Mekk2b)
  • Tag / Fusion Protein
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site N/A (unknown if destroyed)
  • 3′ cloning site N/A (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Alternative plasmid name: pcDNA MEKK2

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA Myc-mMEKK2 was a gift from Xin Lin (Addgene plasmid # 44158 ; http://n2t.net/addgene:44158 ; RRID:Addgene_44158)

  • For your References section:

    Restoration of NF-kappaB activation by tumor necrosis factor alpha receptor complex-targeted MEKK3 in receptor-interacting protein-deficient cells. Blonska M, You Y, Geleziunas R, Lin X. Mol Cell Biol. 2004 Dec;24(24):10757-65. 10.1128/MCB.24.24.10757-10765.2004 PubMed 15572679
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