pcDNA3.1 Myc-huMEKK3 (K391A)
(Plasmid #44157)

• Depositing Lab: Xin Lin
• Publication: Blonska et al Mol Cell Biol. 2004 Dec;24(24):10757-65.

Backbone

• Vector backbone: pcDNA3.1(+)
• Total vector size (bp): 7300
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MEKK3 (K391A)
• Alt name: mitogen-activated protein kinase kinase kinase 3
• Species: H. sapiens (human)
• Insert Size (bp): 1800
• Mutation: K391A, kinase-dead mutant
• Entrez Gene: MAP3K3 (a.k.a. MAPKKK3, MEKK3)
• Tag / Fusion Protein:
  • Myc (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: N/A (unknown if destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Alternative plasmid name: pcDNA3.1 MEKK3 (K391A). MEKK3 K391A, kinase-dead mutant, variant of Addgene plasmid #44156. Mutant of MEKK3 was generated by overlap PCR.