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Addgene

p7013 MSCV-IP N-HAonly 45E6nonsplice
(Plasmid #44146)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44146 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    MSCV-IP N-HAonly
  • Backbone size w/o insert (bp) 8100
  • Total vector size (bp) 8600
  • Modifications to backbone
    Retroviral vector for HA-tagged HPV45 E6 expression. No starting ATG in viral ORF, translation begins from tag only. Puro resistance protein expressed from an IRES.
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HPV45E6
  • Species
    HPV45
  • Insert Size (bp)
    500
  • Mutation
    Internal splice site mutated.
  • Promoter MSCV LTR
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    From DKFZ.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

DKFZ is the co-provider of this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p7013 MSCV-IP N-HAonly 45E6nonsplice was a gift from Peter Howley (Addgene plasmid # 44146 ; http://n2t.net/addgene:44146 ; RRID:Addgene_44146)

  • For your References section:

    Comprehensive analysis of host cellular interactions with human papillomavirus E6 proteins identifies new E6 binding partners and reflects viral diversity. White EA, Kramer RE, Tan MJ, Hayes SD, Harper JW, Howley PM. J Virol. 2012 Dec;86(24):13174-86. doi: 10.1128/JVI.02172-12. Epub 2012 Sep 26. 10.1128/JVI.02172-12 PubMed 23015706
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