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Purpose(Empty Backbone)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
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| Plasmid | 44118 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA4-TO
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Backbone manufacturerInvitrogen
- Backbone size (bp) 6253
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Modifications to backboneFluorescent protein mVenus was modified by insertion of the His8-SBP-FLAG tandem tag and then inserted as a NotI/XbaI fragment into pcDNA4-TO-Puromycin vector, which had the Zeocin selection marker in pcDNA4/TO (Invitrogen) replaced by Puromycin.
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Vector typeMammalian Expression
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Selectable markersPuromycin
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Tag
/ Fusion Protein
- mVenus-MAP (C terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA4-TO-Puromycin-mVenus-MAP was a gift from Dannel McCollum (Addgene plasmid # 44118 ; http://n2t.net/addgene:44118 ; RRID:Addgene_44118) -
For your References section:
A highly efficient multifunctional tandem affinity purification approach applicable to diverse organisms. Ma H, McLean JR, Chao LF, Mana-Capelli S, Paramasivam M, Hagstrom KA, Gould KL, McCollum D. Mol Cell Proteomics. 2012 Aug;11(8):501-11. doi: 10.1074/mcp.O111.016246. Epub 2012 Apr 3. 10.1074/mcp.O111.016246 PubMed 22474084
Map uploaded by the depositor.
