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Addgene

pLX-V5-TRAF2 S11A-puro
(Plasmid #44112)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44112 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLEX970
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Recommended growth media: Terrific broth (TB)
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRAF2 S11A
  • Alt name
    TRAF2
  • Alt name
    TNF receptor-associated factor 2
  • Species
    H. sapiens (human)
  • Mutation
    Serine 11 to Alanine
  • GenBank ID
    NM_021138.3 NP_066961.2
  • Entrez Gene
    TRAF2 (a.k.a. MGC:45012, RNF117, TRAP, TRAP3)
  • Tag / Fusion Protein
    • V5 (C terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: Addgene's quality control sequencing identified an additional P459S mutation. This substitution is not thought to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLX-V5-TRAF2 S11A-puro was a gift from William Hahn (Addgene plasmid # 44112 ; http://n2t.net/addgene:44112 ; RRID:Addgene_44112)

  • For your References section:

    IkappaB kinase epsilon phosphorylates TRAF2 to promote mammary epithelial cell transformation. Shen RR, Zhou AY, Kim E, Lim E, Habelhah H, Hahn WC. Mol Cell Biol. 2012 Dec;32(23):4756-68. doi: 10.1128/MCB.00468-12. Epub 2012 Sep 24. 10.1128/MCB.00468-12 PubMed 23007157
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