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Addgene

pEBB-3XMyc-TRAF2 S408A
(Plasmid #44109)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44109 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEBB
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TRAF2 S408A
  • Alt name
    TRAF2
  • Alt name
    TNF receptor-associated factor 2
  • Species
    H. sapiens (human)
  • Mutation
    Serine 408 to Alanine
  • Entrez Gene
    TRAF2 (a.k.a. MGC:45012, RNF117, TRAP, TRAP3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site BamH1 (not destroyed)
  • 5′ sequencing primer TRAF2 Fwd
  • 3′ sequencing primer M13
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEBB-3XMyc-TRAF2 S408A was a gift from William Hahn (Addgene plasmid # 44109 ; http://n2t.net/addgene:44109 ; RRID:Addgene_44109)

  • For your References section:

    IkappaB kinase epsilon phosphorylates TRAF2 to promote mammary epithelial cell transformation. Shen RR, Zhou AY, Kim E, Lim E, Habelhah H, Hahn WC. Mol Cell Biol. 2012 Dec;32(23):4756-68. doi: 10.1128/MCB.00468-12. Epub 2012 Sep 24. 10.1128/MCB.00468-12 PubMed 23007157
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