pEBB-3XMyc-TRAF2 S11A
(Plasmid
#44105)
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 44105 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepEBB
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameTRAF2 S11A
-
Alt nameTRAF2
-
Alt nameTNF receptor-associated factor 2
-
SpeciesH. sapiens (human)
-
MutationSerine 11 to Alanine
-
GenBank IDNM_021138.3 NP_066961.2
-
Entrez GeneTRAF2 (a.k.a. MGC:45012, RNF117, TRAP, TRAP3)
-
Tag
/ Fusion Protein
- 3xMyc (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamH1 (not destroyed)
- 3′ cloning site BamH1 (not destroyed)
- 5′ sequencing primer TRAF2 Fwd
- 3′ sequencing primer M13-fwd20 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pEBB-3XMyc-TRAF2 S11A was a gift from William Hahn (Addgene plasmid # 44105 ; http://n2t.net/addgene:44105 ; RRID:Addgene_44105) -
For your References section:
IkappaB kinase epsilon phosphorylates TRAF2 to promote mammary epithelial cell transformation. Shen RR, Zhou AY, Kim E, Lim E, Habelhah H, Hahn WC. Mol Cell Biol. 2012 Dec;32(23):4756-68. doi: 10.1128/MCB.00468-12. Epub 2012 Sep 24. 10.1128/MCB.00468-12 PubMed 23007157