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pcDNA4-TO-Hygromycin-sfGFP-MAP
(Plasmid #44100)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44100 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA4-TO
  • Backbone manufacturer
    Invitrogen
  • Backbone size (bp) 7000
  • Modifications to backbone
    Fluorescent proteins sfGFP was modified by insertion of the His8-SBP-FLAG tandem tag and then inserted as a NotI/XbaI fragment into pcDNA4-TO-Hygromycin vector, which had the Zeocin selection marker in pcDNA4/TO (Invitrogen) replaced by hygromycin (Kindly provided by Eric Campeau in University of Massachusetts Medical School).
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin
  • Tag / Fusion Protein
    • sfGFP-MAP (C terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA4-TO-Hygromycin-sfGFP-MAP was a gift from Dannel McCollum (Addgene plasmid # 44100 ; http://n2t.net/addgene:44100 ; RRID:Addgene_44100)

  • For your References section:

    A highly efficient multifunctional tandem affinity purification approach applicable to diverse organisms. Ma H, McLean JR, Chao LF, Mana-Capelli S, Paramasivam M, Hagstrom KA, Gould KL, McCollum D. Mol Cell Proteomics. 2012 Aug;11(8):501-11. doi: 10.1074/mcp.O111.016246. Epub 2012 Apr 3. 10.1074/mcp.O111.016246 PubMed 22474084
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