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pcDNA-HA-Peli1 (mouse)
(Plasmid #44037)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44037 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA-HA
  • Backbone manufacturer
    Shao-Cong Sun
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    pellino 1
  • Alt name
    Peli1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Peli1 (a.k.a. RP23-93O11.1, 2810468L03Rik, A930031K15Rik, AA409794, AI586297, D11Ertd676e)
  • Tag / Fusion Protein
    • 1xHA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-Rev
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pCDNA-HA backbone: Inserted influenza hemagglutinin (HA) epitope tag (YPYDVPDYA) with the translation initiation codon (ATG) into pcDNA3.1 vector (Invitrogen); described in Harhaj, et al. (1999) JBC, 274, 22911–22914.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-HA-Peli1 (mouse) was a gift from Shao-Cong Sun (Addgene plasmid # 44037 ; http://n2t.net/addgene:44037 ; RRID:Addgene_44037)

  • For your References section:

    Peli1 facilitates TRIF-dependent Toll-like receptor signaling and proinflammatory cytokine production. Chang M, Jin W, Sun SC. Nat Immunol. 2009 Oct;10(10):1089-95. doi: 10.1038/ni.1777. Epub 2009 Sep 6. 10.1038/ni.1777 PubMed 19734906
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