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pcDNA-HA-hTRAF3 1-448
(Plasmid #44033)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 44033 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA-HA
  • Backbone manufacturer
    Shao-Cong Sun
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TNF receptor-associated factor 3
  • Alt name
    TRAF3
  • Species
    H. sapiens (human)
  • Mutation
    Contains amino acids 1-448; V274D and S393G
  • Entrez Gene
    TRAF3 (a.k.a. CAP-1, CAP1, CD40bp, CRAF1, IIAE5, LAP1, RNF118)
  • Tag / Fusion Protein
    • 1xHA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-Rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pCDNA-HA backbone: Inserted influenza hemagglutinin (HA) epitope tag (YPYDVPDYA) with the translation initiation codon (ATG) into pcDNA3.1 vector (Invitrogen); described in Harhaj, et al. (1999) JBC, 274, 22911–22914.

Please note that the hTRAF3 insert in this plasmid contains V274D and S393G mutations when compared to GenBank entry NP_663777.1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-HA-hTRAF3 1-448 was a gift from Shao-Cong Sun (Addgene plasmid # 44033 ; http://n2t.net/addgene:44033 ; RRID:Addgene_44033)

  • For your References section:

    Regulation of the NF-kappaB-inducing kinase by tumor necrosis factor receptor-associated factor 3-induced degradation. Liao G, Zhang M, Harhaj EW, Sun SC. J Biol Chem. 2004 Jun 18;279(25):26243-50. Epub 2004 Apr 14. 10.1074/jbc.M403286200 PubMed 15084608
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