pENTR pA L3-L2
(Plasmid
#43966)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 43966 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR221-P3P2
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2557
- Total vector size (bp) 2780
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Vector typeMultisite Gateway ENTR clone
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepA
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Alt namepoly A
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Insert Size (bp)223
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR pA L3-L2 was a gift from Peter Hohenstein (Addgene plasmid # 43966 ; http://n2t.net/addgene:43966 ; RRID:Addgene_43966) -
For your References section:
A universal vector for high-efficiency multi-fragment recombineering of BACs and knock-in constructs. Dolt KS, Lawrence ML, Miller-Hodges E, Slight J, Thornburn A, Devenney PS, Hohenstein P. PLoS One. 2013 Apr 29;8(4):e62054. doi: 10.1371/journal.pone.0062054. Print 2013. 10.1371/journal.pone.0062054 PubMed 23637962