pENTR puroR R4-R3
(Plasmid
#43965)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 43965 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDONR221-P4rP3r
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2670
- Total vector size (bp) 3270
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepuroR
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Alt namepuromycin -R
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Alt namePAC
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Insert Size (bp)600
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR puroR R4-R3 was a gift from Peter Hohenstein (Addgene plasmid # 43965 ; http://n2t.net/addgene:43965 ; RRID:Addgene_43965) -
For your References section:
A universal vector for high-efficiency multi-fragment recombineering of BACs and knock-in constructs. Dolt KS, Lawrence ML, Miller-Hodges E, Slight J, Thornburn A, Devenney PS, Hohenstein P. PLoS One. 2013 Apr 29;8(4):e62054. doi: 10.1371/journal.pone.0062054. Print 2013. 10.1371/journal.pone.0062054 PubMed 23637962