pCR3-FLAG-Ubc13-WT
(Plasmid #43938)

• Depositing Lab: Bryant Darnay
• Publication: Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12. Epub 2006 Nov 29.

Backbone

• Vector backbone: pCR3
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 5540
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ubiquitin-conjugating enzyme E2N
• Alt name: UBC13
• Alt name: UBE2N
• Species: H. sapiens (human)
• Insert Size (bp): 520
• GenBank ID: NM_003348.3
• Entrez Gene: UBE2N (a.k.a. HEL-S-71, UBC13, UBCHBEN, UBCHBEN; UBC13, UbcH-ben, UbcH13)
• Tag / Fusion Protein:
  • 2x FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: BGH Reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry