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Addgene

pCR3-FLAG-Ubc13-WT
(Plasmid #43938)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 43938 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR3
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 5540
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ubiquitin-conjugating enzyme E2N
  • Alt name
    UBC13
  • Alt name
    UBE2N
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    520
  • GenBank ID
    NM_003348.3
  • Entrez Gene
    UBE2N (a.k.a. HEL-S-71, UBC13, UBCHBEN, UBCHBEN; UBC13, UbcH-ben, UbcH13)
  • Tag / Fusion Protein
    • 2x FLAG (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer BGH Reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCR3-FLAG-Ubc13-WT was a gift from Bryant Darnay (Addgene plasmid # 43938 ; http://n2t.net/addgene:43938 ; RRID:Addgene_43938)

  • For your References section:

    Site-specific Lys-63-linked tumor necrosis factor receptor-associated factor 6 auto-ubiquitination is a critical determinant of I kappa B kinase activation. Lamothe B, Besse A, Campos AD, Webster WK, Wu H, Darnay BG. J Biol Chem. 2007 Feb 9;282(6):4102-12. Epub 2006 Nov 29. 10.1074/jbc.M609503200 PubMed 17135271
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