p3XFLAG-TAK1-K63A
(Plasmid
#43933)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 43933 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep3xFLAG-CMV-14
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Backbone manufacturerSigma
- Backbone size w/o insert (bp) 6190
- Total vector size (bp) 8010
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTransforming growth factor-beta-activated kinase 1
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Alt nameTAK1
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Alt nameMAP3K7
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SpeciesM. musculus (mouse)
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Insert Size (bp)1817
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MutationK63A
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GenBank IDNM_172688.3
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Entrez GeneMap3k7 (a.k.a. B430101B05, Tak1)
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Tag
/ Fusion Protein
- 3XFLAG (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer hGH-PA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p3XFLAG-TAK1-K63A was a gift from Bryant Darnay (Addgene plasmid # 43933 ; http://n2t.net/addgene:43933 ; RRID:Addgene_43933) -
For your References section:
TAK1-dependent signaling requires functional interaction with TAB2/TAB3. Besse A, Lamothe B, Campos AD, Webster WK, Maddineni U, Lin SC, Wu H, Darnay BG. J Biol Chem. 2007 Feb 9;282(6):3918-28. Epub 2006 Dec 8. 10.1074/jbc.M608867200 PubMed 17158449