pCR3-FLAG-TRAF6-C70A
(Plasmid #43926)

• Depositing Lab: Bryant Darnay
• Publication: Lamothe et al J Biol Chem. 2007 Feb 9;282(6):4102-12. Epub 2006 Nov 29.

Backbone

• Vector backbone: pCR3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 6690
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TNF Receptor-Associated Factor 6
• Alt name: TRAF6
• Species: M. musculus (mouse)
• Insert Size (bp): 1650
• Mutation: C70A
• GenBank ID: NM_009424.2
• Entrez Gene: Traf6 (a.k.a. 2310003F17Rik, C630032O20Rik)
• Tag / Fusion Protein:
  • 2x FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: BGH Reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: H. Nakano
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry