pCAG-LplA(AILR,36)-NRX3b D137A
(Plasmid #43920)

• Depositing Lab: Alice Ting
• Publication: Liu et al PLoS One. 2013;8(2):e52823. doi: 10.1371/journal.pone.0052823. Epub 2013 Feb 14.

Backbone

• Vector backbone: pCAG
• Backbone size w/o insert (bp): 4247
• Total vector size (bp): 6647
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: AILR-LplA36-NRX3b
• Alt name: Neurexin3b
• Alt name: LplA
• Species: H. sapiens (human), Synthetic
• Mutation: D137A
• Entrez Gene: lplA (a.k.a. b4386, ECK4378, slr, yjjF)
• Promoter: Chicken Beta Actin
• Tag / Fusion Protein:
  • 2XHA

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: CGCCGCCGTCCCCTTCTCCATCTC
• 3′ sequencing primer: CAGGCTTTACACTTTATGCTTCCGGC

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry