MAP2-GCaMP3
(Plasmid #43917)

• Depositing Lab: John Gearhart
• Publication: Addis et al PLoS One. 2011;6(12):e28719. doi: 10.1371/journal.pone.0028719. Epub 2011 Dec 9.

Backbone

• Vector backbone: FU-MAP2-Gateway
• Backbone manufacturer: Addgene plasmid 43915
• Backbone size w/o insert (bp): 9600
• Total vector size (bp): 10900
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Use recA cells, such as STBL3 (Invitrogen)
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GCaMP3
• Alt name: G-CaMP3
• Species: Synthetic
• Insert Size (bp): 1300
• Mutation: E247V in GCaMP3
• GenBank ID: HM143847.1 ADJ53338.1
• Promoter: Human MAP2 promoter
• Tags / Fusion Proteins:
  • 6xHis (N terminal on insert)
  • T7 epitope (N terminal on insert)
  • Xpress tag (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: WPRE-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: GCaMP3 cloned from G-CaMP3, Addgene plasmid 22692.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The MAP2-GFP lentiviral reporter plasmid pGZ-hMAP2 was purchased from System Biosciences (SR10047PA-1). The MAP2 promoter sequence from this plasmid was then cloned along with the Gateway cassette of pEF-DEST51 into the backbone of FUdeltaGW-rtTA (Addgene plasmid 19780) following removal of the ubiquitin promoter-rtTA sequence to produce FU-MAP2-Gateway (Addgene plasmid #43915).

This destination vector was subsequently used to generate MAP2-GCaMP3 (GCaMP3 cloned from G-CaMP3, Addgene plasmid 22692).

The V5 and His tags present in the vector backbone are not expressed with the GCaMP3 insert.