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Addgene

FU-MAP2-Gateway
(Plasmid #43915)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 43915 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    FUdeltaGW-rtTA
  • Backbone manufacturer
    Addgene plasmid 19780
  • Backbone size (bp) 9600
  • Modifications to backbone
    Ubiquitin promoter-rtTA sequence removed. Human MAP2 promoter sequence from pGZ-hMAP2 and Gateway casette from pEF-DEST51 inserted.
  • Vector type
    Mammalian Expression, Lentiviral ; Gateway Destination vector
  • Promoter Human MAP2 promoter
  • Selectable markers
    Zeo marker is outside the LTRs and will not be packaged into virus.
  • Tags / Fusion Proteins
    • V5 (C terminal on backbone)
    • non-standard His tag (HHRHHH) (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    Use ccdb-resistant cells, such as OneShot Survival ccdb Competent Cells (Invitrogen)
  • Copy number
    High Copy

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Backbone plasmid FUdeltaGW-rtTA from Addgene (Plasmid #19780, Konrad Hochedlinger lab); Human MAP2 promoter sequence from plasmid pGZ-hMAP2 (SR10047PA-1) from System Biosciences (Mountain View, CA); Gateway cassette from pEF-DEST51 from Invitrogen.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The MAP2-GFP lentiviral reporter plasmid pGZ-hMAP2 was purchased from System Biosciences (SR10047PA-1). The MAP2 promoter sequence from this plasmid was then cloned along with the Gateway cassette of pEF-DEST51 into the backbone of FUdeltaGW-rtTA (Addgene plasmid 19780) following removal of the ubiquitin promoter-rtTA sequence to produce FU-MAP2-Gateway.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FU-MAP2-Gateway was a gift from John Gearhart (Addgene plasmid # 43915 ; http://n2t.net/addgene:43915 ; RRID:Addgene_43915)

  • For your References section:

    Efficient conversion of astrocytes to functional midbrain dopaminergic neurons using a single polycistronic vector. Addis RC, Hsu FC, Wright RL, Dichter MA, Coulter DA, Gearhart JD. PLoS One. 2011;6(12):e28719. doi: 10.1371/journal.pone.0028719. Epub 2011 Dec 9. 10.1371/journal.pone.0028719 PubMed 22174877
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