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Addgene

pDL86
(Plasmid #43909)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 43909 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS416
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4898
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    DIF1
  • Species
    S. cerevisiae (budding yeast)
  • Entrez Gene
    DIF1 (a.k.a. YLR437C)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Cla1 (unknown if destroyed)
  • 3′ cloning site Xba1 (unknown if destroyed)
  • 5′ sequencing primer M13_forward20_primer
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The following mutations were found during the Addgene QC sequencing: Q31 deletion, S60G and Q68H.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDL86 was a gift from Stephen Elledge (Addgene plasmid # 43909 ; http://n2t.net/addgene:43909 ; RRID:Addgene_43909)

  • For your References section:

    Dif1 is a DNA-damage-regulated facilitator of nuclear import for ribonucleotide reductase. Lee YD, Wang J, Stubbe J, Elledge SJ. Mol Cell. 2008 Oct 10;32(1):70-80. doi: 10.1016/j.molcel.2008.08.018. 10.1016/j.molcel.2008.08.018 PubMed 18851834
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