pcDNAFLAG-ATM-kd
(Plasmid #43908)

• Depositing Lab: Stephen Elledge
• Publication: Cortez et al Science. 1999 Nov 5. 286(5442):1162-6.

Backbone

• Vector backbone: pcDNAFLAG
• Backbone size w/o insert (bp): 5446
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ataxia telangiectasia mutated
• Alt name: ATM
• Alt name: HGNC:795
• Alt name: Entrez Gene: 472
• Species: H. sapiens (human)
• Mutation: kinase dead (D2870A and N2875K)
• Entrez Gene: ATM (a.k.a. AT1, ATA, ATC, ATD, ATDC, ATE, TEL1, TELO1)
• Tags / Fusion Proteins:
  • FLAG (N terminal on insert)
  • His (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry