pCS2-USP44-HIS
(Plasmid
#43894)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 43894 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonep142
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameUSP44
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Alt nameHGNC:20064
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SpeciesH. sapiens (human)
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Entrez GeneUSP44
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Tags
/ Fusion Proteins
- HIS (N terminal on backbone)
- HIS (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Fse1 (unknown if destroyed)
- 3′ cloning site Asc1 (unknown if destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2-USP44-HIS was a gift from Stephen Elledge (Addgene plasmid # 43894 ; http://n2t.net/addgene:43894 ; RRID:Addgene_43894) -
For your References section:
Anaphase initiation is regulated by antagonistic ubiquitination and deubiquitination activities. Stegmeier F, Rape M, Draviam VM, Nalepa G, Sowa ME, Ang XL, McDonald ER 3rd, Li MZ, Hannon GJ, Sorger PK, Kirschner MW, Harper JW, Elledge SJ. Nature. 2007 Apr 19;446(7138):876-81. 10.1038/nature05694 PubMed 17443180