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Addgene

pCS2-USP44
(Plasmid #43893)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 43893 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p138
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    USP44
  • Alt name
    HGNC:20064
  • Species
    H. sapiens (human)
  • Entrez Gene
    USP44
  • Promoter SP6 or T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Fse1 (unknown if destroyed)
  • 3′ cloning site Asc1 (unknown if destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene QC sequencing has found a T91A mutation that is not thought to effect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2-USP44 was a gift from Stephen Elledge (Addgene plasmid # 43893 ; http://n2t.net/addgene:43893 ; RRID:Addgene_43893)

  • For your References section:

    Anaphase initiation is regulated by antagonistic ubiquitination and deubiquitination activities. Stegmeier F, Rape M, Draviam VM, Nalepa G, Sowa ME, Ang XL, McDonald ER 3rd, Li MZ, Hannon GJ, Sorger PK, Kirschner MW, Harper JW, Elledge SJ. Nature. 2007 Apr 19;446(7138):876-81. 10.1038/nature05694 PubMed 17443180
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