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Addgene

MLM2585
(Plasmid #43890)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 43890 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    unknown
  • Backbone manufacturer
    Joung lab
  • Vector type
    TALE Transcriptional Activator Expression Vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TALE-activator p65 backbone
  • Alt name
    RVD of C-terminal 0.5 TALE repeat: NN (binds G)
  • Promoter EF1alpha

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer OMM718 (5’-ATGACGCAGTTCGGGATGAG-3’)
  • 3′ sequencing primer OSQT38 (5’-TTCGGGAATACGGCGATTG-3’)
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Location of plasmid features:
EF1a = 922-2106
start codon = 2119-2121
NLS = 2122-2165
N-term = 2166-2577
0.5 domain = 2592-2658
C-term = 2665-2949
p65 = 2959-3768
pA = 3775-3894

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MLM2585 was a gift from Keith Joung (Addgene plasmid # 43890 ; http://n2t.net/addgene:43890 ; RRID:Addgene_43890)

  • For your References section:

    Robust, synergistic regulation of human gene expression using TALE activators. Maeder ML, Linder SJ, Reyon D, Angstman JF, Fu Y, Sander JD, Joung JK. Nat Methods. 2013 Feb 10. doi: 10.1038/nmeth.2366. 10.1038/nmeth.2366 PubMed 23396285
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