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Addgene

sZF2
(Plasmid #43833)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 43833 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDisplay
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    sZF2
  • Species
    Synthetic
  • Insert Size (bp)
    315
  • Promoter CMV
  • Tags / Fusion Proteins
    • HA Flag (N terminal on insert)
    • Myc (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site SacII (not destroyed)
  • 5′ sequencing primer CMV_F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    sZF2 was a gift from George Church (Addgene plasmid # 43833 ; http://n2t.net/addgene:43833 ; RRID:Addgene_43833)

  • For your References section:

    Barcoding cells using cell-surface programmable DNA-binding domains. Mali P, Aach J, Lee JH, Levner D, Nip L, Church GM. Nat Methods. 2013 Mar 17. doi: 10.1038/nmeth.2407. 10.1038/nmeth.2407 PubMed 23503053
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