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Addgene

Active Wnt10B-V5
(Plasmid #43823)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 43823 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.2/V5-DEST
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7711
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    WNT10B
  • Species
    H. sapiens (human)
  • Entrez Gene
    WNT10B (a.k.a. SHFM6, STHAG8, WNT-12)
  • Promoter CMV
  • Tag / Fusion Protein
    • V5 (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Active Wnt10B-V5 was a gift from Xi He (Addgene plasmid # 43823 ; http://n2t.net/addgene:43823 ; RRID:Addgene_43823)

  • For your References section:

    Disulfide Bond Requirements for Active Wnt Ligands. MacDonald BT, Hien A, Zhang X, Iranloye O, Virshup DM, Waterman ML, He X. J Biol Chem. 2014 May 19. pii: jbc.M114.575027. 10.1074/jbc.M114.575027 PubMed 24841207
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